Penicillin-binding protein genotyping of penicillin-nonsusceptible <i>Streptococcus pneumoniae</i> isolates from the nasopharynx of healthy preschool children

Abstract Streptococcus pneumoniae is one of the most frequent bacterial identified causes community-acquired pneumonia, otitis media and meningitis. It is, as well, a common cause bacteraemia’s significant morbidity mortality. Beta-lactam antibiotics (BLAs) are first line empirical treatment for pneumococcal infections. The targets BLAs penicillin-binding proteins (PBPs), modifications which reasons why pneumococci non-susceptible to BLAs. In our work, total 39 isolates were obtained from 176 healthy children, both vaccinated non-vaccinated. tested antimicrobial susceptibility, their (PBPs) typed by restriction fragment length polymorphism analysis pbp genes. serotypes among penicillin (PNSSP) 23B, 35B 19F. Restriction enzyme analyses pbp1 a, pbp2 b, x genes revealed 5, 3 different patterns, respectively, 4 PBPs profiles PNSSP belonging not included in conjugate vaccines demonstrated. We conclude that level resistance should be monitored constantly ascertain effect current vaccines, well recognize new circumstances developing Poland, possibility multiple, independent imports resistant strains abroad.